Using the SOLO for PCR Automation
نویسنده
چکیده
The polymerase chain reaction (PCR) is one of the most widely used techniques in the field of molecular biology. PCR makes it possible to produce useable quantities of any particular sequence of DNA by systematic amplification of a single sample. The basic process involves mixing the template sequence with a collection of the four deoxyribosenucleotides and DNA polymerase along with a starting primer sequence, salts and buffer. The sample is put in a thermocycler and the temperature is varied through a cycle of three steps:
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